High-Throughput Antibody Discovery Using Barcode Enabled Antigen Mapping (BEAM

Abstract Multi-analyte single cell technologies have potential to greatly accelerate antibody discovery by identifying antigen-specific B cells. Barcode Enabled Antigen Mapping (BEAM) provides a high-throughput, multimodal analysis of antigen-bound cells (BEAM-Ab) using 10x Genomics Chromium Single Cell Immune Profiling v2 Solution and novel computational approaches discover B-cell receptor (BCR) sequences for further functional characterization. To demonstrate the antigen sensitivity BEAM-Ab, we spiked in 5% Hen Egg Lysozyme (HEL) gp120 transgenic 90% wild type non-transgenic splenocytes. The were screened panel barcoded antigens then CD19+PE+ (antigen+) isolated flow cytometry. gene expression, BCR, barcode indicated clonotype specificity respective sorted Furthermore, demonstrated BEAM-Ab analyzing convalescent COVID patient sample against an containing five different negative control. Analysis this suggests clonal expansion recognizing ectodomain SARS-CoV2 spike protein, binding domain D614G mutant protein. We did not observe any Omicron-specific or non-specific control our dataset. Our data demonstrates that is effective tool. unlocks ability rapidly screen large numbers samples with accurate resolution specificity, entire workflow generating candidate just one week after processing.